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  1. 紀要
  2. 第07巻

Two-Step Cellulose Hydrolysis Using Modest Amount of Concentrated Sulfuric Acid or Autoclave and Fermentation of the Hydrolyzates into Ethanol Utilizing Recombinant Yeast

http://hdl.handle.net/10173/534
http://hdl.handle.net/10173/534
0fd7d85f-ced2-4b7a-98a0-5c8dcee1cbc1
名前 / ファイル ライセンス アクション
rb7_101-108.pdf rb7_101-108.pdf (814.8 kB)
Item type 紀要論文 / Departmental Bulletin Paper(1)
公開日 2010-09-03
タイトル
タイトル Two-Step Cellulose Hydrolysis Using Modest Amount of Concentrated Sulfuric Acid or Autoclave and Fermentation of the Hydrolyzates into Ethanol Utilizing Recombinant Yeast
言語
言語 eng
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
資源タイプ departmental bulletin paper
著者 Uryu, Toshiyuki

× Uryu, Toshiyuki

WEKO 4013

Uryu, Toshiyuki

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Katsuraya, Kaname

× Katsuraya, Kaname

WEKO 4014

Katsuraya, Kaname

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Okuyama, Kohsaku

× Okuyama, Kohsaku

WEKO 4015

Okuyama, Kohsaku

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Kato, Hironori

× Kato, Hironori

WEKO 4016

Kato, Hironori

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Uchiyama, Chihiro

× Uchiyama, Chihiro

WEKO 4017

Uchiyama, Chihiro

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Oka, Aiko

× Oka, Aiko

WEKO 4018

Oka, Aiko

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Kubo, Natsumi

× Kubo, Natsumi

WEKO 4019

Kubo, Natsumi

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Takahashi, Mari

× Takahashi, Mari

WEKO 4020

Takahashi, Mari

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Kudo, Mihoko

× Kudo, Mihoko

WEKO 4021

Kudo, Mihoko

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Kawabe, Masataka

× Kawabe, Masataka

WEKO 4022

Kawabe, Masataka

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Kobayashi, Takayuki

× Kobayashi, Takayuki

WEKO 4023

Kobayashi, Takayuki

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Ohnishi, Shiro

× Ohnishi, Shiro

WEKO 4024

Ohnishi, Shiro

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Aida, Mariko

× Aida, Mariko

WEKO 4025

Aida, Mariko

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Shishikura, Kaori

× Shishikura, Kaori

WEKO 4026

Shishikura, Kaori

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Iefuji, Haruyuki

× Iefuji, Haruyuki

WEKO 4027

Iefuji, Haruyuki

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Kobiro, Kazuya

× Kobiro, Kazuya

WEKO 4028

Kobiro, Kazuya

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Tsutsui, Yasukata

× Tsutsui, Yasukata

WEKO 4029

Tsutsui, Yasukata

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Yoshida, Takashi

× Yoshida, Takashi

WEKO 4030

Yoshida, Takashi

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著者別名
姓名 瓜生, 敏之
著者別名
姓名 鬘谷, 要
著者別名
姓名 奥山, 光作
著者別名
姓名 加藤, 裕紀
著者別名
姓名 内山, 千尋
著者別名
姓名 岡, 愛子
著者別名
姓名 久保, 菜津美
著者別名
姓名 高橋, 真梨
著者別名
姓名 工藤, 美穂子
著者別名
姓名 川辺, 雅敬
著者別名
姓名 小林, 崇幸
著者別名
姓名 大西, 史朗
著者別名
姓名 相田, 真理子
著者別名
姓名 宍倉, 香
著者別名
姓名 家藤, 治幸
著者別名
姓名 小廣, 和哉
著者別名
姓名 筒井, 康賢
著者別名
姓名 吉田, 孝
抄録
内容記述タイプ Abstract
内容記述 Tissue paper was hydrolyzed by the two-step acidic hydrolysis using 60-80 wt.% of concentrated sulfuric acid in the first step to produce hydrolyzates containing glucose as a main component. The hydrolyzate was fermented by means of ß-glucosidases-producing recombinant yeast to afford ethanol in the maximum yield of 7.7%. In the use of such modest amount of concentrated sulfuric acid, repetition of the two-step hydrolysis was effective. From wood powder, ethanol was obtained in the maximum yield of 6.9%. When autoclave heating at 190-200ºC was carried out as the first-step hydrolysis
instead of concentrated sulfuric acid, the maximum ethanol yield was 2.7% and 1.0% from tissue paper and wood powder, respectively. These results indicate that mainly amorphous cellulose on the surface can be hydrolyzed by such amount of concentrated sulfuric acid and that thermomechanical operation might be necessary for hydrolyzing crystalline cellulose.
抄録
内容記述タイプ Abstract
内容記述 ティッシュペーパーを第一段階で60-80 wt.% 量の濃硫酸を使用する二段階酸加水分解で加水分解し、グルコースを主成分とする加水分解物を生成させた。加水分解物は、β-グルコシダーゼ産生組換え酵母によって発酵され、最高収率7.7%でエタノールを与えた。この中程度量の濃硫酸を使用する時、二段階加水分解の繰返し操作が有効であった。木粉からは最高収率6.9%でエタノールが生成した。190-200ºCにおけるオートクレーブ加熱を濃硫酸の代りに第一段階加水分解に使用すると、最高エタノール収率は、ティッシュペーパーと木粉からそれぞれ2.7%および1.0%であった。これらの結果は、表面に存在する主にアモルファスセルロースがそのような量の濃硫酸によって加水分解されること、および高温における機械的操作が結晶セルロースの加水分解には必須であることを示している。
書誌情報 高知工科大学紀要

巻 7, 号 1, p. 101-108, 発行日 2010-07-29
ISSN
収録物識別子タイプ ISSN
収録物識別子 1348-4842
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA11954573
著者版フラグ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
その他のタイトル
その他のタイトル 中程度量の濃硫酸又はオートクレーブを使用する二段階加水分解と組換え酵母を利用する加水分解物のエタノール発酵
出版者
出版者 高知工科大学
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