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  1. 学術雑誌論文

Robust expression of heterologous genes by selection marker fusion system in improved Chlamydomonas strains

http://hdl.handle.net/10173/1464
http://hdl.handle.net/10173/1464
53c84870-7f94-4ec6-914e-3c65768c1c35
名前 / ファイル ライセンス アクション
28-32.pdf 28-32.pdf (747.9 kB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2017-03-24
タイトル
タイトル Robust expression of heterologous genes by selection marker fusion system in improved Chlamydomonas strains
言語
言語 eng
キーワード
主題Scheme Other
主題 Transgene silencing
キーワード
主題Scheme Other
主題 Position effect
キーワード
主題Scheme Other
主題 Squalene synthase-like gene
キーワード
主題Scheme Other
主題 Botryococcus braunii
キーワード
主題Scheme Other
主題 Methyltransferase gene
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
資源タイプ journal article
著者 Kong, Fantao

× Kong, Fantao

WEKO 1193

Kong, Fantao

Search repository
Yamasaki, Tomohito

× Yamasaki, Tomohito

WEKO 1194

Yamasaki, Tomohito

Search repository
Kurniasih, Sari Dewi

× Kurniasih, Sari Dewi

WEKO 1195

Kurniasih, Sari Dewi

Search repository
Hou, Liyuan

× Hou, Liyuan

WEKO 1196

Hou, Liyuan

Search repository
Li, Xiaobo

× Li, Xiaobo

WEKO 1197

Li, Xiaobo

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Ivanova, Nina

× Ivanova, Nina

WEKO 1198

Ivanova, Nina

Search repository
Okada, Shigeru

× Okada, Shigeru

WEKO 1199

Okada, Shigeru

Search repository
Ohama, Takeshi

× Ohama, Takeshi

WEKO 1200

Ohama, Takeshi

Search repository
抄録
内容記述タイプ Abstract
内容記述 Chlamydomonas is a very attractive candidate plant cell factory. However, its main drawback is the difficulty to find the transformants that robustly express heterologous genes randomly inserted in the nuclear genome. We previously showed that domestic squalene synthase (SQS) gene of Chlamydomonas was much more efficiently overexpressed in a mutant strain [UV-mediated mutant (UVM) 4] than in wild type. In this study, we evaluated the possibility of a new mutant strain, met1, which contains a tag in the maintenance type methyltransferase gene that is expected to play a key role in the maintenance of transcriptional gene silencing. The versatile usefulness of the UVM4 strain to express heterologous genes was also analyzed. We failed to overexpress CrSSL3 cDNA, which is the codon-adjusted squalene synthase-like gene originated from Botryococcus braunii, using the common expression cassette in the wild-type CC-1690 and UVM4 strains. However, we succeeded in isolating western blot-positive transformants through the combinational use of the UVM4 strain and ble2A expression system of which expression cassette bears a fused ORF of the target gene and the antibiotic resistance gene ble via the foot-and-mouth disease virus (FMDV) self-cleaving 2A sequence. It is noteworthy that even with this system, huge deviations in the accumulated protein levels were still observed among the UVM4 transformants.
書誌情報 Journal of Bioscience and Bioengineering

巻 120, 号 3, p. 239-245, 発行日 2015-09
ISSN
収録物識別子タイプ ISSN
収録物識別子 1389-1723
DOI
関連タイプ isVersionOf
識別子タイプ DOI
関連識別子 10.1016/j.jbiosc.2015.01.005
権利
権利情報 © 2015 The Society for Biotechnology, Japan.
著者版フラグ
出版タイプ AM
出版タイプResource http://purl.org/coar/version/c_ab4af688f83e57aa
出版者
出版者 Elsevier B.V.
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