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Effect of an Introduced Phytoene Synthase Gene Expression on Carotenoid Biosynthesis in the Marine Diatom Phaeodactylum tricornutum
http://hdl.handle.net/10173/1481
http://hdl.handle.net/10173/14813b0c7fbf-68a9-4d7c-9b05-e083a6efdda1
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
28-33.pdf (748.5 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2017-03-24 | |||||
| タイトル | ||||||
| タイトル | Effect of an Introduced Phytoene Synthase Gene Expression on Carotenoid Biosynthesis in the Marine Diatom Phaeodactylum tricornutum | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | microalgae | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | marine diatom | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | transformation | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | PSY | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | carotenoid | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Kadono, Takashi
× Kadono, Takashi× Kira, Nozomu× Suzuki, Kengo× Iwata, Osamu× Ohama, Takeshi× Okada, Shigeru× Nishimura, Tomohiro× Akakabe, Mai× Tsuda, Masashi× Adachi, Masao |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Carotenoids exert beneficial effects on human health through their excellent antioxidant activity. To increase carotenoid productivity in the marine Pennales Phaeodactylum tricornutum, we genetically engineered the phytoene synthase gene (psy) to improve expression because RNA-sequencing analysis has suggested that the expression level of psy is lower than other enzyme-encoding genes that are involved in the carotenoid biosynthetic pathway. We isolated psy from P. tricornutum, and this gene was fused with the enhanced green fluorescent protein gene to detect psy expression. After transformation using the microparticle bombardment technique, we obtained several P. tricornutum transformants and confirmed psy expression in their plastids. We investigated the amounts of PSY mRNA and carotenoids, such as fucoxanthin and β-carotene, at different growth phases. The introduction of psy increased the fucoxanthin content of a transformants by approximately 1.45-fold relative to the levels in the wild-type diatom. However, some transformants failed to show a significant increase in the carotenoid content relative to that of the wild-type diatom. We also found that the amount of PSY mRNA at log phase might contribute to the increase in carotenoids in the transformants at stationary phase. | |||||
| 書誌情報 |
Marine Drugs 巻 13, 号 8, p. 5334-5357, 発行日 2015-08 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1660-3397 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.3390/md13085334 | |||||
| 権利 | ||||||
| 権利情報 | This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0). | |||||
| 権利（URI） | ||||||
| 権利情報 | http://creativecommons.org/licenses/by/4.0/ | http://creativecommons.org/licenses/by/4.0/ | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 出版者 | ||||||
| 出版者 | MDPI | |||||
