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  1. 学術雑誌論文

Characterization of marine diatom-infecting virus promoters in the model diatom Phaeodactylum tricornutum

http://hdl.handle.net/10173/1496
http://hdl.handle.net/10173/1496
7a4bb04f-b764-4bf6-9a30-2ca31854a265
名前 / ファイル ライセンス アクション
28-34.pdf 28-34.pdf (1.2 MB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2017-03-24
タイトル
タイトル Characterization of marine diatom-infecting virus promoters in the model diatom Phaeodactylum tricornutum
言語
言語 eng
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
資源タイプ journal article
著者 Kadono, Takashi

× Kadono, Takashi

WEKO 1434

Kadono, Takashi

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Miyagawa-Yamaguchi, Arisa

× Miyagawa-Yamaguchi, Arisa

WEKO 1435

Miyagawa-Yamaguchi, Arisa

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Kira, Nozomu

× Kira, Nozomu

WEKO 1436

Kira, Nozomu

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Tomaru, Yuji

× Tomaru, Yuji

WEKO 1437

Tomaru, Yuji

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Okami, Takuma

× Okami, Takuma

WEKO 1438

Okami, Takuma

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Yoshimatsu, Takamichi

× Yoshimatsu, Takamichi

WEKO 1439

Yoshimatsu, Takamichi

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Hou, Liyuan

× Hou, Liyuan

WEKO 1440

Hou, Liyuan

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Ohama, Takeshi

× Ohama, Takeshi

WEKO 1441

Ohama, Takeshi

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Fukunaga, Kazunari

× Fukunaga, Kazunari

WEKO 1442

Fukunaga, Kazunari

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Okauchi, Masanori

× Okauchi, Masanori

WEKO 1443

Okauchi, Masanori

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Yamaguchi, Haruo

× Yamaguchi, Haruo

WEKO 1444

Yamaguchi, Haruo

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Ohnishi, Kohei

× Ohnishi, Kohei

WEKO 1445

Ohnishi, Kohei

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Falciatore, Angela

× Falciatore, Angela

WEKO 1446

Falciatore, Angela

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Adachi, Masao

× Adachi, Masao

WEKO 1447

Adachi, Masao

Search repository
抄録
内容記述タイプ Abstract
内容記述 Viruses are considered key players in phytoplankton population control in oceans. However, mechanisms that control viral gene expression in prominent microalgae such as diatoms remain largely unknown. In this study, potential promoter regions isolated from several marine diatom-infecting viruses (DIVs) were linked to the egfp reporter gene and transformed into the Pennales diatom Phaeodactylum tricornutum. We analysed their activity in cells grown under different conditions. Compared to diatom endogenous promoters, novel DIV promoter (ClP1) mediated a significantly higher degree of reporter transcription and translation. Stable expression levels were observed in transformants grown under both light and dark conditions, and high levels of expression were reported in cells in the stationary phase compared to the exponential phase of growth. Conserved motifs in the sequence of DIV promoters were also found. These results allow the identification of novel regulatory regions that drive DIV gene expression and further examinations of the mechanisms that control virus-mediated bloom control in diatoms. Moreover, the identified ClP1 promoter can serve as a novel tool for metabolic engineering of diatoms. This is the first report describing a promoter of DIVs that may be of use in basic and applied diatom research.
書誌情報 Scientific Reports

巻 5, p. 18708-1-18708-13, 発行日 2015-12
ISSN
収録物識別子タイプ ISSN
収録物識別子 2045-2322
DOI
関連タイプ isIdenticalTo
識別子タイプ DOI
関連識別子 10.1038/srep18708
権利
権利情報 This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
権利(URI)
権利情報 http://creativecommons.org/licenses/by/4.0/ | http://creativecommons.org/licenses/by/4.0/
著者版フラグ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
出版者
出版者 Nature Publishing Group
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